重組干細胞因子(SCF)在干細胞維持與組織發(fā)育研究中的作用機制
重組干細胞因子（Stem Cell Factor, SCF，AbMole，M9986）是一種多效性細胞因子，通過特異性結(jié)合酪氨酸激酶受體c-KIT（CD117），觸發(fā)受體二聚化并激發(fā)激酶活性，進而調(diào)控細胞增殖、凋亡、分化和遷移等關(guān)鍵生物學(xué)過程[1]。SCF/c-KIT結(jié)合后可激活多種信號通路，包括磷脂酰肌醇3-激酶-蛋白激酶B（PI3K-AKT）、Janus激酶/信號轉(zhuǎn)導(dǎo)與轉(zhuǎn)錄激活因子（JAK-STAT）、絲裂原活化蛋白激酶（MAPK）等。重組干細胞因子（Stem Cell Factor, SCF，AbMole，M9986）常與其他細胞因子聯(lián)合用于細胞的培養(yǎng)或者動物發(fā)育研究等實驗。研究顯示，CD34⁺造血干細胞在含重組SCF、IL-3（重組白介素3蛋白，Interleukin-3, P08700）和Flt3-L（重組FMS樣胰激酶3配體，P49771）的培養(yǎng)基中，可在體外維持長期增殖能力，且保留多向分化潛能[2, 3]。重組SC（uniprot: P21583-1）在TF-1細胞系（人紅白血病細胞）中，通過激活PI3K-AKT通路顯著促進細胞增殖[4]。
此外，在動物模型中，SCF通過c-KIT介導(dǎo)的MAPK/ERK通路調(diào)控大鼠腹膜肥大細胞（RPMCs）的過敏反應(yīng)[5]。在轉(zhuǎn)基因CADASIL模型（TgNotch3R90C小鼠）中，重組SCF聯(lián)合G-CSF（重組粒細胞集落刺激因子，P09920）可減輕腦小血管血栓形成，改善血管平滑肌細胞退化及認知功能[6]。重組SCF局部注射至新生小鼠卵巢可非侵入性促進卵泡激活與發(fā)育[7]；大鼠胚胎皮層神經(jīng)元實驗進一步揭示，SCF（重組干細胞因子，Stem Cell Factor）可通過MEK/ERK/p53信號通路增強神經(jīng)突觸延伸[8]。2014年，AbMole的兩款抑制劑分別被西班牙國家心血管研究中心和美國哥倫比亞大學(xué)用于動物體內(nèi)實驗，相關(guān)科研成果發(fā)表于頂刊 Nature 和 Nature Medicine。
*本文所述產(chǎn)品僅供科研使用
參考文獻及鳴謝
[1] Cardoso, H. J.; Figueira, M. I.; Socorro, S. The stem cell factor (SCF)/c-KIT signalling in testis and prostate cancer. Journal of cell communication and signaling 2017, 11 (4), 297-307.
[2] Cicuttini, F.; Begley, C.; Boyd, A. J. G. F. The effect of recombinant stem cell factor (SCF) on purified CD34-positive human umbilical cord blood progenitor cells. 1992, 6 (1), 31-39.
[3] Shen, B.; Jiang, W.; Fan, J.; et al. Residues 39-56 of stem cell factor protein sequence are capable of stimulating the expansion of cord blood CD34+ cells. 2015, 10 (10), e0141485.
[4] Mazzoldi, E. L.; Pavan, S.; Pilotto, G.; et al. A juxtacrine/paracrine loop between C-Kit and stem cell factor promotes cancer stem cell survival in epithelial ovarian cancer. Cell death & disease 2019, 10 (6), 412.
[5] Nam, S. Y.; Kim, H. Y.; Kim, H. M.; et al. Betaeta-eudesmol reduces stem cell factor-induced mast cell migration. International immunopharmacology 2017, 48, 1-7.
[6] Ping, S.; Qiu, X.; Gonzalez-Toledo, M. E.; et al. Stem Cell Factor in Combination With Granulocyte Colony-Stimulating Factor Protects the Brain From Capillary Thrombosis-Induced Ischemic Neuron Loss in a Mouse Model of CADASIL. Frontiers in cell and developmental biology 2020, 8, 627733.
[7] Wang, Y.; Zhang, J.; Liang, J.; et al. In vivo promotion of primordial follicle activation by stem cell factor treatment in mice with premature ovarian insufficiency and advanced age. Molecular human reproduction 2022, 29 (1).
[8] Gao, M.; Zhao, L. R. Turning Death to Growth: Hematopoietic Growth Factors Promote Neurite Outgrowth through MEK/ERK/p53 Pathway. Molecular neurobiology 2018, 55 (7), 5913-5925.
此外，在動物模型中，SCF通過c-KIT介導(dǎo)的MAPK/ERK通路調(diào)控大鼠腹膜肥大細胞（RPMCs）的過敏反應(yīng)[5]。在轉(zhuǎn)基因CADASIL模型（TgNotch3R90C小鼠）中，重組SCF聯(lián)合G-CSF（重組粒細胞集落刺激因子，P09920）可減輕腦小血管血栓形成，改善血管平滑肌細胞退化及認知功能[6]。重組SCF局部注射至新生小鼠卵巢可非侵入性促進卵泡激活與發(fā)育[7]；大鼠胚胎皮層神經(jīng)元實驗進一步揭示，SCF（重組干細胞因子，Stem Cell Factor）可通過MEK/ERK/p53信號通路增強神經(jīng)突觸延伸[8]。2014年，AbMole的兩款抑制劑分別被西班牙國家心血管研究中心和美國哥倫比亞大學(xué)用于動物體內(nèi)實驗，相關(guān)科研成果發(fā)表于頂刊 Nature 和 Nature Medicine。
*本文所述產(chǎn)品僅供科研使用
參考文獻及鳴謝
[1] Cardoso, H. J.; Figueira, M. I.; Socorro, S. The stem cell factor (SCF)/c-KIT signalling in testis and prostate cancer. Journal of cell communication and signaling 2017, 11 (4), 297-307.
[2] Cicuttini, F.; Begley, C.; Boyd, A. J. G. F. The effect of recombinant stem cell factor (SCF) on purified CD34-positive human umbilical cord blood progenitor cells. 1992, 6 (1), 31-39.
[3] Shen, B.; Jiang, W.; Fan, J.; et al. Residues 39-56 of stem cell factor protein sequence are capable of stimulating the expansion of cord blood CD34+ cells. 2015, 10 (10), e0141485.
[4] Mazzoldi, E. L.; Pavan, S.; Pilotto, G.; et al. A juxtacrine/paracrine loop between C-Kit and stem cell factor promotes cancer stem cell survival in epithelial ovarian cancer. Cell death & disease 2019, 10 (6), 412.
[5] Nam, S. Y.; Kim, H. Y.; Kim, H. M.; et al. Betaeta-eudesmol reduces stem cell factor-induced mast cell migration. International immunopharmacology 2017, 48, 1-7.
[6] Ping, S.; Qiu, X.; Gonzalez-Toledo, M. E.; et al. Stem Cell Factor in Combination With Granulocyte Colony-Stimulating Factor Protects the Brain From Capillary Thrombosis-Induced Ischemic Neuron Loss in a Mouse Model of CADASIL. Frontiers in cell and developmental biology 2020, 8, 627733.
[7] Wang, Y.; Zhang, J.; Liang, J.; et al. In vivo promotion of primordial follicle activation by stem cell factor treatment in mice with premature ovarian insufficiency and advanced age. Molecular human reproduction 2022, 29 (1).
[8] Gao, M.; Zhao, L. R. Turning Death to Growth: Hematopoietic Growth Factors Promote Neurite Outgrowth through MEK/ERK/p53 Pathway. Molecular neurobiology 2018, 55 (7), 5913-5925.
標簽：
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