彈性蛋白聚合物（ELP）凝聚層分析的新型高通量方法
New High-Throughput Method for Elastin-like Polymer (ELP) Coacervate Analysis
彈性蛋白聚合物（ELP）凝聚層分析的新型高通量方法
Posted by Michelle Devoe
Michelle Devoe發(fā)表
December 2018 — A recent study by researchers from the University of New England and University of New Hampshire has demonstrated that flow imaging microscopy is an accurate, more efficient, and more informative method of elastin-like polymer (ELP) coacervate analysis than standard methods. ELP coacervates are a class of molecules with promising applications in drug delivery vehicles, tissue engineering, environmental remediation, and more. ELP coacervate architecture is stimuli-responsive and highly tunable, making them ideal for the above-mentioned applications.
2018年12月 - 新英格蘭大學(xué)和新罕布什爾大學(xué)的研究人員最近的一項(xiàng)研究表明，與標(biāo)準(zhǔn)方法相比，流動(dòng)成像顯微鏡是一種準(zhǔn)確，更有效，信息更豐富的彈性蛋白樣聚合物（ELP）凝聚分析方法。 ELP凝聚層在作為藥物遞送的載體，組織工程，環(huán)境修復(fù)等方面具有廣闊的應(yīng)用前景。 ELP凝聚層結(jié)構(gòu)具有刺激響應(yīng)性和高度可調(diào)性，使其成為上述應(yīng)用的理想選擇。
ELP coacervates imaged by the FlowCam. Size and circularity, two of the 40+ properties that can be measured by the FlowCam, is used to sort coacervates. Image from Marvin et al. (2018).
ELP凝聚了由FlowCam成像的圖像。 尺寸和圓度是FlowCam可以測(cè)量的40多個(gè)屬性中的兩個(gè)，用于對(duì)凝聚層進(jìn)行分類(lèi)。 圖片來(lái)自Marvin等。（2018）。
Standard methods for ELP coacervate analysis are indirect and cumbersome. Data from Visible-UV spectrophotometer turbidity measurements and dynamic light scattering (DLS) size measurements are superimposed to evaluate the formation of micron-scale aggregates, and particle geometry is constructed indirectly from diffusion data. Imaging analysis methods such as optical, electron, and scanning probe microscopy are useful methods to acquire size and shape data, however it is time-consuming and laborious to acquire a large enough sample size to get statistically significant data.
ELP凝聚層分析的標(biāo)準(zhǔn)方法是間接和麻煩的。 來(lái)自可見(jiàn) - 紫外分光光度計(jì)濁度測(cè)量和動(dòng)態(tài)光散射（DLS）尺寸測(cè)量的數(shù)據(jù)被疊加以評(píng)估微米級(jí)聚集體的形成，并且顆粒幾何形狀間接地由擴(kuò)散數(shù)據(jù)構(gòu)建。 諸如光學(xué)，電子和掃描探針顯微鏡之類(lèi)的成像分析方法是獲取尺寸和形狀數(shù)據(jù)的有用方法，然而獲得足夠大的樣本大小以獲得統(tǒng)計(jì)上有效的數(shù)據(jù)是耗時(shí)且費(fèi)力的。
In this study by the University of New England and University of New Hampshire, the size, morphology, and behavior of ELP coacervates subjected to various solvent conditions were measured and observed using the FlowCam particle analyzer. Results were validated by comparison with DLS and atomic force microscopy analyses. Flow imaging microscopy was demonstrated to be a successful method for ELP coacervate analysis. Additionally, flow imaging microscopy reported additional findings that were not measured using the DLS, microscopy, or Visible-UV spectrophotometry.
在新英格蘭大學(xué)和新罕布什爾大學(xué)的這項(xiàng)研究中，使用FlowCam顆粒分析儀測(cè)量和觀(guān)察經(jīng)受各種溶劑條件的ELP凝聚層的尺寸，形態(tài)和行為。 通過(guò)與DLS和原子力顯微鏡分析的比較驗(yàn)證結(jié)果。 流動(dòng)成像顯微鏡被證明是ELP凝聚層分析的成功方法。 此外，流動(dòng)成像顯微鏡提供了DLS，顯微鏡或可見(jiàn)紫外分光光度法不能獲得的結(jié)果。
彈性蛋白聚合物（ELP）凝聚層分析的新型高通量方法
Posted by Michelle Devoe
Michelle Devoe發(fā)表
December 2018 — A recent study by researchers from the University of New England and University of New Hampshire has demonstrated that flow imaging microscopy is an accurate, more efficient, and more informative method of elastin-like polymer (ELP) coacervate analysis than standard methods. ELP coacervates are a class of molecules with promising applications in drug delivery vehicles, tissue engineering, environmental remediation, and more. ELP coacervate architecture is stimuli-responsive and highly tunable, making them ideal for the above-mentioned applications.
2018年12月 - 新英格蘭大學(xué)和新罕布什爾大學(xué)的研究人員最近的一項(xiàng)研究表明，與標(biāo)準(zhǔn)方法相比，流動(dòng)成像顯微鏡是一種準(zhǔn)確，更有效，信息更豐富的彈性蛋白樣聚合物（ELP）凝聚分析方法。 ELP凝聚層在作為藥物遞送的載體，組織工程，環(huán)境修復(fù)等方面具有廣闊的應(yīng)用前景。 ELP凝聚層結(jié)構(gòu)具有刺激響應(yīng)性和高度可調(diào)性，使其成為上述應(yīng)用的理想選擇。
ELP coacervates imaged by the FlowCam. Size and circularity, two of the 40+ properties that can be measured by the FlowCam, is used to sort coacervates. Image from Marvin et al. (2018).
ELP凝聚了由FlowCam成像的圖像。 尺寸和圓度是FlowCam可以測(cè)量的40多個(gè)屬性中的兩個(gè)，用于對(duì)凝聚層進(jìn)行分類(lèi)。 圖片來(lái)自Marvin等。（2018）。
Standard methods for ELP coacervate analysis are indirect and cumbersome. Data from Visible-UV spectrophotometer turbidity measurements and dynamic light scattering (DLS) size measurements are superimposed to evaluate the formation of micron-scale aggregates, and particle geometry is constructed indirectly from diffusion data. Imaging analysis methods such as optical, electron, and scanning probe microscopy are useful methods to acquire size and shape data, however it is time-consuming and laborious to acquire a large enough sample size to get statistically significant data.
ELP凝聚層分析的標(biāo)準(zhǔn)方法是間接和麻煩的。 來(lái)自可見(jiàn) - 紫外分光光度計(jì)濁度測(cè)量和動(dòng)態(tài)光散射（DLS）尺寸測(cè)量的數(shù)據(jù)被疊加以評(píng)估微米級(jí)聚集體的形成，并且顆粒幾何形狀間接地由擴(kuò)散數(shù)據(jù)構(gòu)建。 諸如光學(xué)，電子和掃描探針顯微鏡之類(lèi)的成像分析方法是獲取尺寸和形狀數(shù)據(jù)的有用方法，然而獲得足夠大的樣本大小以獲得統(tǒng)計(jì)上有效的數(shù)據(jù)是耗時(shí)且費(fèi)力的。
In this study by the University of New England and University of New Hampshire, the size, morphology, and behavior of ELP coacervates subjected to various solvent conditions were measured and observed using the FlowCam particle analyzer. Results were validated by comparison with DLS and atomic force microscopy analyses. Flow imaging microscopy was demonstrated to be a successful method for ELP coacervate analysis. Additionally, flow imaging microscopy reported additional findings that were not measured using the DLS, microscopy, or Visible-UV spectrophotometry.
在新英格蘭大學(xué)和新罕布什爾大學(xué)的這項(xiàng)研究中，使用FlowCam顆粒分析儀測(cè)量和觀(guān)察經(jīng)受各種溶劑條件的ELP凝聚層的尺寸，形態(tài)和行為。 通過(guò)與DLS和原子力顯微鏡分析的比較驗(yàn)證結(jié)果。 流動(dòng)成像顯微鏡被證明是ELP凝聚層分析的成功方法。 此外，流動(dòng)成像顯微鏡提供了DLS，顯微鏡或可見(jiàn)紫外分光光度法不能獲得的結(jié)果。
標(biāo)簽：
顆粒分析儀
- 顯微計(jì)數(shù)法不溶性微粒分析儀養(yǎng)護(hù)清潔指南
- 基于NTA技術(shù)的納米氣泡粒徑時(shí)空演變與表面張力主導(dǎo)機(jī)制研究
- 流式動(dòng)態(tài)圖像法微粒分析儀工作原理及根源破解微粒溯源的應(yīng)用
- 激光粒度分析儀在新藥研發(fā)中加速處方篩選與優(yōu)化的應(yīng)用
- 中國(guó)藥典要求及技術(shù)現(xiàn)狀下制劑不溶性微�？刂频慕鉀Q方案
- PSS納米粒度分析儀ZETA電位分析儀的操作優(yōu)勢(shì)及維護(hù)方法
- NTA 表征技術(shù)賦能納米氣泡：理化特性解析與多領(lǐng)域應(yīng)用進(jìn)展
- 從檢測(cè)原理到儀器選型，不溶性微粒檢測(cè)方法的全攻略梳理
- 德瑞科儀推出不溶性微粒檢測(cè)儀測(cè)樣試用活動(dòng)
- 四度科學(xué)納米顆粒跟蹤分析儀亮相全國(guó)細(xì)胞外囊泡大會(huì)
- 四度科學(xué)自研納米顆粒跟蹤分析儀亮相中草藥囊泡大會(huì)
- 2024慕尼黑分析生化展大昌華嘉科學(xué)儀器部閃耀登場(chǎng)
- 北京世紀(jì)朝陽(yáng)科技發(fā)展有限公司誠(chéng)聘區(qū)域銷(xiāo)售經(jīng)理
- DKSH二手儀器保障服務(wù)-流式顆粒成像系統(tǒng)8000系列
- 大昌華嘉助力重大儀器設(shè)備“以舊換新”—選型指南
- 沃特世推出高效測(cè)量的Zeta電位納米粒度分析儀
Copyright(C) 1998-2026 生物器材網(wǎng) 電話(huà)：021-64166852;13621656896 E-mail：info@bio-equip.com